主题：【原创】这是真的吗？Maxipreps concentration＝4163.1ng/ul？！ -- 大鹏翔宇

Take lane 3 for example: the plasmid is 4045 bp according to the map while the DNA fragment in lane 3 is somewhere around 6k bp. That's why I said you have already put something (a gene) in the vector.

I think you should have since you will use it for transfection. Are you going to use luciferase as a reporter gene?