主题：【原创】这是真的吗？Maxipreps concentration＝4163.1ng/ul？！ -- 大鹏翔宇

Is your vectors empty or you have already put something in it (1.8 - 2k bps?)? It looks to me that the size of DNA fragments in gel are larger than what it should be based on the plamid map.

Usually I would not bother to do it if it's a vector. Reason: 1. If you will use it as stock, only plasmid can transform when you want to amplify it; 2. If you want to use it for ligation, you will purify the fragment after the digestion, which will separate them. However, it may consume some of your enzyme, you need take it into account.